Zero may refer to:
- The S-Class Maverick Hunter Zero from the Megaman X series.
- Zero, the ninja-esque human fighter from Anarchy Reigns.
- Zero, the main protagonist from Drakengard 3.
- Zero-Two, the final boss of Kirby's Dreamland 3 and Kirby 64: The Crystal Shards.
- Zero Kiryu, the main protagonist of Vampire Knight.
- Zer0, a vault hunter from Borderlands 2